Carbachol increases basolateral K+ conductance in T84 cells. Simultaneous measurements of cell [Ca] and gK explore calcium's role.
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Carbachol increases basolateral K+ conductance in T84 cells. Simultaneous measurements of cell [Ca] and gK explore calcium's role
To explore the role of calcium in mediating the action of carbachol in chloride-secreting epithelia, we simultaneously measured intracellular free [Ca] ([Ca]i) and the potassium conductance (gK) of the basolateral membrane in T84 cells grown on collagen-coated filters. [Ca]i was measured with fura-2 and fluorescence microscopy and expressed as a relative value ([Ca]'i) normalized to control. To...
متن کاملCarbachol Increases Basolateral K + Conductance in T 84 Cells
To explore the role of calcium in mediating the action of carbachol in chloride-secreting epithelia, we simultaneously measured intracellular free [Ca] ([Ca].) and the potassium conductance (gx) of the basolateral membrane in T84 cells grown on collagen-coated filters. [Call was measured with fura-2 and fluores-cence microscopy and expressed as a relative value ([Ca][) normalized to control. To...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1990
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.96.6.1271